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Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis . Analysis showed that testosterone and dihydrotestosterone regulated adult hippocampal neurogenesis (AHN). Adult hippocampal neurogenesis was regulated through the androgen receptor in the wild-type male rats, but not in the TMF male rats. To further test the role of activated androgen receptors on AHN, flutamide , an antiandrogen drug that competes with testosterone and dihydrotestosterone for androgen receptors , and dihydrotestosterone were administered to normal male rats. Dihydrotestosterone increased the number of BrdU cells, while flutamide inhibited these cells.

The legal status of anabolic steroids varies from country to country. In the ., anabolic steroids are listed as Schedule III controlled substances under the Controlled Substances Act , which makes the possession of such substances without a prescription a federal crime punishable by up to seven years in prison. [39] In Canada, anabolic steroids and their derivatives are part of the Controlled drugs and substances act and are Schedule IV substances, meaning that it is illegal to obtain or sell them without a prescription. However, possession is not punishable, a consequence reserved for schedule I, II or III substances. Those guilty of buying or selling anabolic steroids in Canada can be imprisoned for up to 18 months. Importing or exporting anabolic steroids also carry similar penalties. [40] Anabolic steroids are also illegal without prescription in Australia, [41] Argentina, Brazil, and Portugal, [42] and are listed as Schedule 4 Controlled Drugs in the United Kingdom.

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